Niemann-Pick C1 protects against atherosclerosis in mice via regulation of macrophage intracellular cholesterol trafficking
J. Clin. Invest. Jessie R. Zhang, et al. 118:2281 doi:10.1172/JCI32561 [
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Figure 1Analysis of BM reconstitution of
Ldlr–/– mice after BMT from
Ldlr–/–Npc1+/+ and
Ldlr–/–Npc1–/– donors.
(
A) Transplantation scheme for generation of
Ldlr–/– mice with BM-derived cells deficient in
Npc1. (
B) At 6 weeks after BMT, peripheral blood was obtained from recipients, and cells were stained with anti-CD45.1 and anti-CD45.2 to identify Ly5.1- and Ly5.2-positive cells by flow cytometry. Data represent 10
4 cells from each mouse. (
C) Cholesterol staining of peritoneal macrophages harvested from high-fat fed Mϕ
Npc1+/+ and Mϕ
Npc1–/– chimeric mice. Arrowhead denotes cholesterol-laden lysosomes. Scale bar: 10 μM.
